In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3
Article Abstract:
All steps of eukaryotic translation in vitro were reconstituted using purified ribosomal subunits, initiation, elongation, and termination factors, and aminoacyl transfer ribonucleic acids. Termination using pretermination complexes were examined assembled on messenger ribonucleic acid (mRNA) encoding a tetra-peptide and a model was proposed for translation termination that accounts for the cooperative action of release factors eRF1 and eRF3 in ensuring fast release of nascent polypeptide.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2006
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Initiation of protein synthesis from the A site of the ribosome
Article Abstract:
The assembly of 80S ribosomes in the cricket paralysis virus involves 40S and 60S ribosomal subunits with a CCU and GCU triplets at the P and the A site respectively. Data indicate that although the A site lacks translation initiation factors, initiation of protein synthesis occurs at this site suggesting involvement of unknown factors.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2000
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Entrapping ribosomes for viral translation: tRNA mimicry as a molecular Trojan hourse
Article Abstract:
Research has been conducted on turnip yellow mosaic virus with a genomic plus-strand RNA. The interactions between turnip yellow mosaic virus-RNA-programmed ribosomes and 3'-valylated tRNA-like structure have been investigated, and the details are reported.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2003
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