In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3

Article Abstract:

All steps of eukaryotic translation in vitro were reconstituted using purified ribosomal subunits, initiation, elongation, and termination factors, and aminoacyl transfer ribonucleic acids. Termination using pretermination complexes were examined assembled on messenger ribonucleic acid (mRNA) encoding a tetra-peptide and a model was proposed for translation termination that accounts for the cooperative action of release factors eRF1 and eRF3 in ensuring fast release of nascent polypeptide.

Science & research, Research, Eukaryotes, RNA, Polypeptides, Genetic research

---

Initiation of protein synthesis from the A site of the ribosome

Article Abstract:

The assembly of 80S ribosomes in the cricket paralysis virus involves 40S and 60S ribosomal subunits with a CCU and GCU triplets at the P and the A site respectively. Data indicate that although the A site lacks translation initiation factors, initiation of protein synthesis occurs at this site suggesting involvement of unknown factors.

United States, Proteins, Protein synthesis, Genetic translation, Translation (Genetics)

---

Entrapping ribosomes for viral translation: tRNA mimicry as a molecular Trojan hourse

Article Abstract:

Research has been conducted on turnip yellow mosaic virus with a genomic plus-strand RNA. The interactions between turnip yellow mosaic virus-RNA-programmed ribosomes and 3'-valylated tRNA-like structure have been investigated, and the details are reported.

Netherlands, Genetic aspects, Viruses, Genomes, Transfer RNA, Cell research, Cytological research

Similar abstracts:

• Abstracts: Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators
• Abstracts: Functional definition of regions necessary for replication and incompatibility in the Mycobacterium fortuitum plasmid pAL5000
• Abstracts: RAG1/2-mediated resolution of transposition intermediates: two pathways and possible consequences. The polymeric immunoglobulin receptor translocates pneumococci across human nasopharyngeal epithelial cells
• Abstracts: Identification and quantification of methanogenic Archaea in adult chicken ceca. Archaeal population dynamics during sequential reduction processes in rice field soil
• Abstracts: The evolution of the G matrix: selection or drift? Natural selection and genetic differentiation of behaviour between parasitoids from wild and cultivated habitats

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.