In vivo production, stabilization, and infectivity of Baculovirus preoccluded virions
Article Abstract:
Preoccluded virus forms (POV) of baculoviruses are more potent in infecting host larvae than wild-type viruses and are easily stabilized and produced in larval tissue. Almost 50% of the potency of the wild-type-virus is due to the occluded polyhedra and the rest is due to the POV in the tissue. The polyhedrin-negative viruses are more infectious than the wild-type and their potency is unaffected by lyophilization. The POVs are unstable in dead larval tissues. The ability of POV to infect larvae opens up possibilities for their use as biopesticides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Methods for detection of Anticarsia gemmatalis nucleopolyhedrovirus DNA in soil
Article Abstract:
Baculoviruses are double-stranded, circular DNAs that only infect insects. These viruses are sometimes genetically altered to control certain types of pests from destroying agricultural products and crops. However, there are instances when baculovirus DNA remain on the soil. A method of detecting baculovirus DNA in the soil involves the extraction of humic acids from the soil through sequential incubation in sodium pyrophosphate and sodium hydroxide and followed by successive phenol-ether extractions to purify the viral DNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Colonization pattern of the biocontrol strain Pseudomonas chlororaphis MA 342 on barley seeds visualized by using green fluorescent protein
Article Abstract:
Pseudomonas chlororaphis colocalizes with the fungal pathogen Drechslera teres on seeds of cereal crops. The bacterium can be used as a biocontrol agent for this fungus, which causes net blotch of barley.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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- Abstracts: Characterization of a novel strain of Bacillus thuringiensis. Cloning of a novel cryIC-type gene from a strain of Bacillus thuringiensis subsp. galleriae
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