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Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway

Article Abstract:

The study attempted to increase production of carotenoid pigments in the food yeast Candida utilis. This was done by applying concepts of metabolic engineering to the yeast's isoprenoid pathway. Carbon flux was then redirected away from the ergosterol formation for utilization by the carotenoid pathway. A sevenfold increase in the yield of the red carotenoid lycopene was noted in the C. utilis strain. In addition, other carotenoids such as beta-carotene and astaxanthin were also produced.

Author: Fraser, Paul D., Misawa, Norihiko, Shimada, Hiroshi, Kondo, Keiji, Miura, Yutaka, Saito, Toshiko
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
Physiological aspects, Carotenoids, Yeast fungi, Microbial biotechnology, Yeasts (Fungi), Carotenoid metabolism

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Expression of the psl operon in pseudomonas aeruginosa PAO1 biofilms: PsIA performs an essential function in biofilm formation

Article Abstract:

The regulation of the psl gene cluster and the function of the first gene in the pslA gene cluster are investigated in biofilm formation and on the regulation of the entire psl operon in planktonic and biofilm cells. It is suggested that constitutive expression of the psl operon enables efficient attachment to surfaces and that regulated localized psl operon expression is required for biofilm differentiation.

Author: Rehm, Bernd H.A., Webb, Jeremy S., Overhage, Jorg, Schemionek, Mirle
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Science & research, Analysis, Genetic aspects, Operons, Pseudomonas aeruginosa, Biosynthesis, Nucleic acids, Nucleic acid isolation

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Subjects list: Research
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