Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting

Article Abstract:

Switch recombination, or Cre-loxP recombination system of bacteriophage P1, occurs between Ig (immunoglobin gene) switch region (S region) sequences, and involves the alignment of two partner S sequences (S mu and S region) of a downstream C region gene and the deletion by looping out of the intervening chromosomal DNA between 2 partner S region sequences on the same chromosome. The regulation of S gamma 1 rearrangement in switch recombination is independent of the S mu rearrangement and the E mu element on the same chromosome.

Observations, Genetic recombination

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Immunoglobulin heavy and light chain genes rearrange independently at early stages of B cell development

Article Abstract:

A study of the assembly of immunoglobulin genes during the early development of B cells is presented. The study protocol involved the resolution of mouse B cell progenitors into five developmentally related fractions using multicolor flow cytometry. The results showed that expression of the pre-B cell receptor complex is needed for the transition from the large CD43+ to the small CD43- pre-B cell stage. Furthermore, immunoglobulin heavy and light chain gene rearrangement is initiated in the large CD43+ pre-B cell stage.

Physiological aspects, Immunoglobulins, B cells

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Loss of a gp130 cardiac muscle cell survival pathway is a critical event in the onset of heart failure during biomechanical stress

Article Abstract:

The critical role of a myocyte survival pathway in the transition between compensatory cardiac hypertrophy and heart failure is demonstrated. Using Cre-lox technology to obtain a ventricular chamber-restricted knockout of the gp130 cytokine receptor, it is proven that cardiac myocyte apoptosis is the critical point in the transition, leading to the conclusion that the use of ligands operating via gp130 may represent a novel strategy for preventing heart failure.

Muscle cells, Cytokines, Heart failure

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