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Biological sciences

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Influence of environmental parameters on phosphatidylcholine phospholipase C production in Listeria monocytogenes: a convenient method to differentiate L. monocytogenes from other Listeria species

Article Abstract:

The increase in phosphatidylcholine phospholipase C (lecithinase) production by changing environmental factors is an efficient method to distinguish Listeria monocytogenes from other Listeria species. The production of lecithinase is dependent on virulence in the pathogenic species of Listeria. L. monocytogenes degrades lecithin while other Listeria species are unable to do so. The optimal conditions for lecithinase production are, a pH between 7 and 7.3, a temperature of 37 to 40 degrees Celsius and 1.75 to 2% NaCl.

Environmental aspects, Virulence (Microbiology), Listeria monocytogenes

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Homologous expression of the lipase and ABC transporter gene cluster, tliDEFA, enhances lipase secretion in Pseudomonas spp

Article Abstract:

Pseudomonas fluorescens containing the ABC transporter tliDEFA could be used to produce lipase for industrial uses. Lipases are used in detergents, in the processing of fats, in organic synthesis, and in the chiral resolution of racemic mixtures.

Microbial biotechnology, Pseudomonas fluorescens, Lipase, Biotechnological microorganisms

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Simultaneous enhancement of thermostability and catalytic activity of phospholipase A(sub 1) by evolutionary molecular engineering

Article Abstract:

Researchers were able to increase the thermostability of phospholipase A from Serratia sp. Strain MK1 using evolutionary molecular engineering. This technique is used to optimize the performance of enzymes used in industrial applications.

Enzymes

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