Influence of temperature and growth phase on expression of a 104-kilodalton Listeria adhesion protein in Listeria monocytogenes

Article Abstract:

The interaction of Listeria monocytogenes with mammalian intestinal cells contributes to Listeria pathogenesis. A 104-kDa surface protein called Listeria adhesion protein was found to be involved in adhesion of Listeria monocytogenes to a human enterocyte-like Caco-2 cell line based on transposon mutagenesis. The expression of Listeria adhesion protein in Listeria monocytogenes was also analyzed at different growth phases and temperatures using an enzyme-linked immunoassay and Western blotting with a specific monoclonal antibody.

Physiological aspects, Bacteria, Bacterial adhesion, Proteins, Gene expression, Listeria monocytogenes, Temperature, Listeria

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Cytotoxicity potential and genotypic characterization of Escherichia coli isolates from environmental and food sources

Article Abstract:

The presence of virulence gene markers in 1,698 environmental isolates of Escherichia coli and 81 isolates from food and clinical sources is examined by using multiplex PCR. The results suggest that the multiple virulence factors typically used to identify potentially virulent Escherichia coli strains are insufficient for distinguish cytopathic isolates.

Science & research, Genotype, Genotypes, Escherichia coli, Genetic markers, Virulence (Microbiology)

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