Introduction of anaerobic dechlorinating bacteria into soil slurry microcosms and nested-PCR monitoring

Article Abstract:

The introduction of anaerobic dechlorinating bacteria, Desulfomonile tiedjei and Desulfitobacterium dehalogenans, induces reductive dechlorination activity in nonsterile soil slurry microcosms. Nested-PCR monitoring reveals that the addition of D. tiedjei to microcosms, supplemented with pyruvate, enhances the biotransformation of chlorobenzoate. The addition of D. dehalogenans reduces the period for dechlorination activity and increases the rate of degradation of 3-chloro-4-hydroxy-phenoxyacetic acid.

Analysis, Observations, Microbial metabolism, Soils, Soil analysis, Anaerobic bacteria

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Detection of Dekkera-Brettanomyces strains in sherry by a nested PCR method

Article Abstract:

A Dekkera yeast strain obtained from sherries and other Brettanomyces and Dekkera strains have been genetically characterized. A two-step polymerase chain reaction (PCR) method was developed using a genomic DNA fragment of the isolated Dekkera strain. The total PCR assay takes less than 10 hours to perform and eliminates the need for isolation and culture. The method shows potential forthe sensitive monitoring of Dekkera-Brettanomyces strains.

Yeast fungi, Yeasts (Fungi), Sherry

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Mitochondrial DNA loss caused by ethanol in Saccharomyces flor yeasts

Article Abstract:

Ethanol directly damages the mitochondrial membrane in Saccharomyces flor yeasts, destroying the mitochondrial DNA (mtDNA). However, ethanol is unlikely to be a direct mutagenic agent for mtDNA polymorphism in flor yeasts. It influences long-term evolution of the yeast mitochondrial genome. The mtDNAs may face a positive selection pressure relating to their ability to tolerate the effect of ethanol.

Physiological aspects, Alcohol, Ethanol, Mitochondrial DNA, Yeast, Yeast (Food product)

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