Investigation of persistent colonization by Pseudomonas aeruginosa-like strains in a spring water bottling plant

Article Abstract:

Computer-assisted whole-cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was utilized to determine the origin and persistence of Pseudomonas aeruginosa-like strains. SDS-PAGE analysis of the bacterial strains isolated from a spring water bottling plant resulted in the formation of four clusters at the similarity level of 80%. Persistent colonization was caused by a single strain with two clonal origins that are 99.3% similar to Pseudomonas alcaligenes.

Usage, Identification and classification, Gel electrophoresis, Taxonomy (Biology), Microbial populations, Bacteriology

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Exploitation of gene involved in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a Pseudomonas strain

Article Abstract:

The involvement of 2,4-diacetylphloroglucinol (Phl) in the capacity of Pseudomonas sp. strain F113to act as a biocontrol agent on sugar beet seeds in nonsterilized soil is studied. A Phl production-negative mutant was partially complemented by a recombinant plasmid containing this region. The ability of Pseudomonas strain in increasing the emergence of sugar beet seeds involving these genes was demonstrated.

Biosynthesis

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Selection of a Pseudomonas cepacia strain constitutive for the degradation of trichloroethylene

Article Abstract:

A technique for the production of a nonrecombinant Pseudomonas cepacia strain capable of oxidizing Trichloroethylene is presented.This involves the cloning of three toluene oxygenases in Escherichia coli underalternate promoter control. One mutant strain was found to exhibit a completelystable constitutive degradative phenotype through 100 generations of nonselective growth.

Research, Biodegradation, Trichloroethylene

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