Lacticin 3147, a broad-spectrum bacteriocin which selectively dissipates the membrane potential

Article Abstract:

Partial purification by chromatography was used to characterize lacticin 3147, a bacteriocin produced by Lactococcus lactis. Results revealed that two components of the bacteriocin are required for full activity. The bacteriocin was bactericidal against L. lactis, Listeria monocytogenes and Bacillus subtilis, while bactericidal activity was enhanced when target cells were energized. These suggested that the presence of a proton motive force promotes the interaction of lacticin 3147 with the cytoplasmic membrane.

Antibacterial agents, Bacterial proteins

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Characterization and heterologous expression of the genes encoding enterocin A production, immunity, and regulation in Enterococcus faecium DPC1146

Article Abstract:

Enterocin A production is inducible in Enterococcus faecium DPC1146. An inverse PCR method based on a core sequence comprised of the enterocin A structural and immunity genes successfully obtained and sequenced some 11 kb of the genome of E faecium DPC1146. Genetic information required for production of enterocin A in a heterologous strain is contained in this locus. Several new open reading frames were also identified, some of which may play a role in either regulation or processing and export of bacteriocins.

Physiological aspects, Bacteria

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Identification of aerobically and anaerobically induced genes in Enterococcus faecalis by random arbitrarily primed PCR

Article Abstract:

The random arbitrarily primed polymerase chain reaction (RAP-PCR) method was adapted and optimized to allow its reproducible use in studies of gene expression by a gram-positive bacterium. It has previously been broadly applied in the analysis of patterns of eukaryotic gene expression. After several modifications, it was used in a reproducible manner in the study of differential enterococcal gene expression.

Methods, Polymerase chain reaction

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