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Linear correlation between bacterial overexpression of recombinant peptides and cell light scatter

Article Abstract:

A linear correlation has been observed between cell forward-angle light scattering and the level of bacterial overexpression of atrial natriuretic peptide. The presence of fusion peptides enhances either forward-angle light scattering or right-angle light scattering. This correlation is faster and an effective way for optimizing peptide production. It is protein product specific and is seen only in unlysed cells.

Author: Cenatiempo, Y., Lavergne-Mazeau, F., Maftah, A., Julien, R.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
Analysis, Physiological aspects, Bacterial cell walls, Recombinant proteins, Light scattering

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Biological activities adn structural properties of the atypical bacteriocins mesenterocin 52B and leucocin B-TA33a

Article Abstract:

The bacteriocins mesenterocin 52B and leucocin B-TA33a have different antibacterial spectra even though they have high sequence homology and adopt the same conformation in aqueous lipophilic micellar systems. They also appear to be different from class IIa bacteriocins and from bacteriocins of other subgroups.

Author: Corbier, C., Krier, F., Mulliert, G., Vitoux, B., Revol-Junelles, A.-M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001

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Method for rapid purification of class IIa bacteriocins and comparison of their activities

Article Abstract:

Researchers describe a three-step method they used to purify mesentericin Y105 from Leuconostoc mesenteroides Y105 and five other anti-Listeria bacteriocins. The method consists of affinity chromatography on blue agarose, ultrafiltration, and then reverse-phase high-pressure liquid chromatography.

Author: Guyonnet, D., Fremaux, C., Cenatiempo, Y., Berjeaud, J.M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000

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Subjects list: Research, Bacterial toxins
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