Loss of O157 O antigenicity of verotoxin-producing Escherichia coli O157:H7 surviving under starvation conditions

Article Abstract:

Assays for E. coli O157:H7 that are based on the O157 O antigen may not detect the bacterium if it has spent long periods of time under starvation conditions. Researchers show under these conditions, the bacterium loses the O antigen but still produces verotoxin.

Author: Hara-Kudo, Yukiko, Miyahara, Michiko, Kumagai, Susumu
Antigenic determinants

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Enterohemorrhagic Escherichia coli O157:H7 present in radish sprouts

Article Abstract:

Research was conducted to investigate the presence of viable enterohemorrhagic Escherichia coli O157:H7 in the inner tissues and stomata of cotyledons of radish sprouts. The objective was to determine if E coli O157:H7, which was considered a nonphytophathogenic bacterium, could be present in a culturable form in radish sprouts grown from seeds contaminated with the bacterium. Results indicate that E coli O157:H7 was present not only on outer surfaces but also in inner tissues of cotyledons and stomata of radish sprouts.

Author: Hara-Kudo, Yukiko, Kumagai, Susumu, Konuma, Hirotaka, Itoh, Yoshinori, Sugita-Konishi, Yoshiko, Kasuga, Fumiko, Iwaki, Masaaki, Saito, Noriko, Nogushi, Yoko
Radishes

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Effects of starvation on physiological activity and chlorine disinfection in Escherichia coli O157:H7

Article Abstract:

A study was conducted to investigate the influence of starvation on physiological activity and chlorine disinfection resistance in Escherichia coli O157:H7 using fluorogenic strains and probes. Results indicated that starvation supported the development of an injury-resistant membrane structure against deoxycholate susceptibility during the absence of disinfection. Findings also showed that intracellular mechanisms accommodate disinfection resistance.

Author: McFeters, Gordon A., Pyle, Barry H., Lisle, John T., Broadaway, Susan S., Prescott, Annette M., Fricker, Colin
Chlorine

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Subjects list: Research, Escherichia coli, Starvation
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