Marker systems for the phenotypic recognition of maternally derived trisomics in maize

Article Abstract:

Marker systems, developed for maize, utilize B-A translocation and allow phenotypic recognition of different doses of chromosome arm transmitted through the female parent. The markers are transposons that show dosage effects on the timing and rate of transposition of a responder in anthocyanin pigment gene. The transposons affecting anthocyanin pigment gene are incorporated into the B-A translocations. Crossing protocols and stocks are developed, which allow phenotypic detection of extra chromosomes or chromosomal segments. The system can be used to detect trisomics.

Author: Birchler, J.A., Guo, M.
Transposons

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Marker systems for B-A translocations in maize

Article Abstract:

A system for genetic dosage analysis for corn involving the multicopy chromosome B and the single copy chromosome A is presented. The system is a unique method in mapping genes and determination of copy numbers. A B to A translocation marker system in 14 chromosome arms is discussed in terms of dosage class identification at the mature seed state. The system will not only be a useful tool in mapping of genes and the determination of the number of copies but in the characterization of mutants and maintaining A to B translocations as well.

Author: Birchler, J.A., Alfenito, M.R.
Genetic aspects, Translocation (Genetics)

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Chromosomal location of eight isozyme loci in rice using primary trisomics and monosomic alien addition lines

Article Abstract:

Polymorphism and chromosomal location of isozyme loci in rice (Oryza sativa L.) was detected using starch-gel electrophoresis and isoelectric focusing techniques. Four new isozyme loci polymorphism were detected, with primary trisomic analysis showing that Dia-1 and Fk-1 are sited on chromosome 1, Mdh-1 on chromosome 5 and Fk-1, with Gdh-2 and Tpi-1 observed as a cluster on chromosome 11.

Author: Delos Reyes, B.G., Khush, G.S., Brar, D.S.
Rice, Electrophoresis

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Subjects list: Research, Usage, Observations, Corn, Trisomy
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