Mei-W68 in Drosophila melanogaster encodes a Spo11 homolog: evidence that the mechanism for initiating meiotic recombination is conserved
Article Abstract:
Meitotic recombination requires activity of several gene products in Drosophila melanogaster and Saccharomyces cerevisiae. Through use of a new genetic mapping method, mei-W68 has been cloned to give high-resolution mapping to a less-than-18-db region with a homolog of the S. cerevisiae spo11 gene. In the new mapping method, P elements are used to promote crossing-over at their insertion sites. Spo11 is proposed as the enzymatic activity that brings the double-strand breaks required to initiate meiotic recombination. Perhaps recombination in Drosophila is started by double-strand breaks. Evidence that the mechanism for initiating meiotic recombination is conserved exists.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
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Rad52 associates with RPA and functions with Rad55 and Rad57 to assemble meiotic recombination complexes
Article Abstract:
Rad52, the Saccharomyces cerevisiae recombination protein, and RPA, the single-strand DNA-binding protein, combine into cytologically detectable subnuclear complexes in recombination in meiosis. Double-strand breaks (DSBs) are not adequate for the appearance of Rad51 foci, the detectable subnuclear complexes. They promote formation of RPA, Rad52 and Rad51 foci.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
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