Membrane adsorption with direct cell culture combined with reverse transcription-PCR as fast method for identifying enteroviruses from sewage

Article Abstract:

A new approach for the detection and identification of enterviruses concentrated and isolated form sewage is presented. Viruses were absorbed to cellulose nitrate membrane filters, cultured directly from the membrane filters by using the VIRADEN method, and identified by reverse transcription-PCR, followed by 5' untranslated region (5'-UTR) restriction fragment length polymorphism (RFLP) analysis and partial sequencing of the VP1 protein coding region.

Genetic polymorphisms, Genetic code

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A protective immune response is generated in rainbow trout by an OompH-like surface antigen (P18) of Flavobacterium psychrophilum

Article Abstract:

An investigation of the surface characteristics of Flavobacterium psychrophilum that is an important pathogen of fish is conducted to enable in identifying a surface protein termed P18, by a two-step method. The purification and characterization of the immunogenic and protective 18-kDa outer membrane-associated OmpH-like protein is reported.

Risk factors, Genetic aspects, Bacterial infections, Rainbow trout, High performance liquid chromatography, Psychrophilic microorganisms

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Antigenic characterization of the fish pathogen Flavobacterium psychrophilum

Article Abstract:

The potential vaccine targets of Flavobacterium psychrophilum are a 16 kDa lipopolysaccharide and a 20 kDa protein. This organism causes rainbow trout fry syndrome and bacterial cold water disease.

Diseases, Fishes, Bacteria, Pathogenic, Pathogenic bacteria

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