Methanobrevibacter ruminantium as an indicator of domesticated-ruminant fecal pollution in surface waters

Article Abstract:

The fecal pollution is detected from domesticated ruminants in environmental water samples by using a PCR-based assay (Mrnif) targeting the nifH gene of Methanobrevibacter ruminantium. The assay is shown to be sensitive and can distinguish between domesticated- and nondomesticated-ruminant fecal pollution in environmental samples.

Waste Management and Remediation Services, Water Pollution, Physiological aspects, Environmental aspects, Methanobacteriaceae, Methanogens, Ruminants

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Multiplex PCR screening to detect cry9 genes in Bacillus thuringiensis strains

Article Abstract:

A PCR method is described that can detect all four known genes in Bacillus thuringiensis that produce insecticidal crystal proteins. This bacterium is a biological control agent that can kill agricultural pests.

Bacillus thuringiensis, Pests, Biological control

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PCR-based strategy to detect and identify species of phaeoacremonium causing grapevine diseases

Article Abstract:

A nested PCR strategy by using general fungal primers ITS1F/ITS4 which was developed to detect Phaeoacremonium diseases directly in grapevine wood and produce plants free of the disease is presented.

Science & research, Research, Fungal diseases of plants, Plant fungal diseases, Primers (Molecular genetics)

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