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Biological sciences

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Methanol oxidation mutants in Methylobacterium extorquens AM1: identification of new genetic complementation groups

Article Abstract:

Complementation groups in the Mxb, Mxc and Mxd gene locus can be identified by analyses of the 208 Methylobacterium extorquens AM1 methanol oxidation mutants. The Mxb and Mxc mutants have low concentrations of the subunit MxaF and cytochrome C(sub L) compared to the wild type. The mutants have a high concentration of cytochrome c-553. The concentration of MxaF and c cytochromes in the MxdR and MxdS mutants is similar to that in the wild-type bacteria. The Mxb and Mxc mutants fail to complement the Mxd mutants.

Research, Bacteria, Complementation (Genetics)

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Molecular characterization of a chromosomal region involved in the oxidation of acetyl-CoA to glyoxylate in the isocitrate-lyase-negative methylotroph Methylobacterium extorquens AM1

Article Abstract:

The characterization of the chromosome region of the mutant isocitrate-lyase-negative methylotroph Methylobacterium extorquens AM1 reveals six complete and two partial ORFs. Mutations in genes encoding a coenzyme-B12-linked mutase, an alcohol dehydrogenase and a propionyl-CoA carboxylase lead to loss of the ability to convert acetyl-CoA to glyoxylate. The mutants are C1- and C2-deficient, and their growth is restored by the addition of glyoxylate or glycolate.

Physiological aspects, Microbial enzymes, Amino acid sequence, Amino acid sequencing

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