Minireplicon from pBtoxis of Bacillus thuringiensis subsp. israelensis

Article Abstract:

A study identified, cloned and sequenced a 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis. This fragment consists of cis elements, including iterons, found in replication origins of other large plasmids and suggests that pBtoxis replicates by a type A theta mechanism.

Science & research, Endotoxins, Structure, DNA replication, Report

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Novel isolate of Bacillus thuringiensis subsp. thuringiensis that produces a quasicuboidal crystal of Cry1Ab21 toxic to larvae of Trichoplusia ni

Article Abstract:

A new isolate (IS5056) of Bacillus thuringiensis that has produced a novel variant of Cry1Ab, Cry1Ab21, is isolated from soil collected in northeastern Poland. The inclusion is composed of a new toxic Cry protein variant, CryAb21, which has invaded Thuringiensis ni and is reproduced by using the larval cadaver as a substrate for vegetative growth and sporulation.

Usage, Environmental aspects, Bacterial toxins, Cladistic analysis, Microbiological assay

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Molecular genetic manipulation of truncated Cry1C protein synthesis in Bacillus thuringiensis to improve stability and yield

Article Abstract:

Results show that synthesis and stability of the truncated Cry1C of the insecticidal Cry proteins can be increased by combining genetic elements from other endotoxin genes and operons in combination with promoters. A transcriptional termination sequence and its orientation are important for stabilization and yield of the truncated protein.

United States, Statistical Data Included, Physiological aspects, Protein synthesis, Protein biosynthesis, Insecticides, Molecular genetics

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