Mitochondrial Mg (super.2+) homeostasis is critical for group II intron splicing in vivo

Article Abstract:

Mitochondrial Mg(super.2+) homeostasis has been found to be critical for in vivo group II intron splicing. The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor required for all group II introns in mitochondria of Saccharomyces cerevisiae. Mutant alleles of the MRS2 gene and overexpression of the gene raise Mg(super.2+) concentration in mitochondria and compensate for splicing defects of certain group II introns.

Austria, Statistical Data Included, Genetic aspects, Mitochondria, Yeast, Yeast (Food product), Introns

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Molecular genetic analysis of the heterodimeric splicing factor U2AF: the RS domain on either the large or small Drosophila subunit is dispensable in vivo

Article Abstract:

Genetic data from tests of Drosophila suggest that U2AF RS domains are not necessary for functions in vivo though they are essential for in vitro. U2AF is a pre-mRNA splicing factor that is important for 3' splice site selection. Repeated tests with differing subunit homologs confirmed the finding that they are redundant for in vivo functioning.

Drosophila

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The carboxyl terminus of vertebrate poly(A) polymerase interacts with U2AF 65 to couple 3'-end processing and splicing

Article Abstract:

Research shows that the carboxyl end of vertebrate poly(A) polymerase works with U2AF 65 to couple 3'-end processing/splicing. The molecular basis of the coupling interaction of pre-mRNA splicing and 3'-end formation was studied. The mechanism by which a cleavage/polyadenylation site stimulates splicing of an adjacent upstream intron was found.

Germany, RNA polymerases, RNA, Scission (Chemistry)

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