Molecular genetic analysis of the region containing the essential Pseudomonas aeruginosa asd gene encoding aspartate-beta-semialdehyde dehydrogenase

Article Abstract:

Polymerase chain reaction (PCR) amplification was utilized to determine the molecular structure and amino acid sequence of the asd gene of Pseudomonas aeruginosa which encodes aspartate-beta-semialdehyde dehydrogenase. PCR analysis of the region containing the asd gene of Pseudomonas aeruginosa indicated a 2433 bp SmaI-Nsil fragment that was composed of a putative orfA gene. Furthermore, the asd gene maps to the same macrorestriction fragments as other genes involved in amino acid biosynthesis.

Research, Polymerase chain reaction, Microbial enzymes, Dehydrogenases, Oxidoreductases, Chromosome mapping

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pilO, a gene required for glycosylation of Pseudomonas aeruginosa 1244 pilin

Article Abstract:

The gene adjacent to the gene coding for pilin formation in Pseudomonas aeruginosa may contribute to the enhancement and activity of pilin. Pilin is encoded in P. aeruginosa by a gene named pilA. The downstream sequence, pilO, exhibits a similar tRNa and transcriptional termination sequence as pilA. Mutants containing only the pilA gene produced a smaller pilin protein which could not be identified using the pilin-reactive reagent typically used to detect pilin.

Pseudomonas

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Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa

Article Abstract:

Transposon mutagenesis results demonstrate that a 2.8 kilobase open reading frame is the fimV gene which codes a peptidoglycan-binding domain along with transmembrane domains and an acidic C terminus. Data further show that fimV gene is responsible for the twiching motility exhibited by Pseudomonas aeruginosa.

United States, Australia, Physiological aspects, Bacteria, Peptidoglycans, Transposons, Bacterial motility

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