Molecular genetics of the Drosophila melanogaster ovo locus, a gene required for sex determination of germline cells

Article Abstract:

The ovo locus of Drosophila melanogaster was cloned and analyzed. The DNA lesions causing three ovo loss-of-function mutations and three svb- ovo- double mutations were identified. The mutations cluster within a 7-kilobase (kb) region that is homologous with the female-specific, germline-dependent transcription unit for a 5-kb poly(A)+ RNA size class. A cloned 10-kb segment that includes the mutant cluster and the transcription unit for the 5-kb RNA size class without a neighboring 6-kb RNA transcription unit can reverse the germline effects of ovo mutations.

Germ cells, Molecular genetics, Sex determination, Genetic, Sex determination (Genetics)

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The transposable element mariner mediates germline trsnsformation in Drosophila melanogaster

Article Abstract:

Researchers used the transportable element mariner to design a vector, called pMIwB, for germline transformation in Drosophila melanogaster. It has a mariner element disrupted by an insertion having the wild-type white gene from D. melanogaster, the beta-galactosidase gene from Escherichia coli and sequences that assist in the plasmid replication and selection in E. coli. The mariner transposable element can enhance the introduction of the big (13.2kb) MIwB element, hinting that mariner may offer a large host-range transformation vector for insects.

Models, Transposons

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Identification of regions interacting with ovo(D) mutations: potential new genes involved in germline sex determination or differentiation in Drosophila melanogaster

Article Abstract:

Two regions in the Drosophila melanogaster germline are identified. Their gene products function in developmental hierarchies. The two regions consist of several germline sex determination genes. The presence of the two regions is identified by the development of new systematic screening techniques.

Microbial mutation

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