Monitoring methanotrophic bacteria in hybrid anaerobic-aerobic reactors with PCR and a catabolic gene probe

Article Abstract:

Research was conducted to examine the ability of methanotrophic bacteria to grow and to survive in small upflow anaerobic sludge bed bioreactors. Monitoring different methanotrophic populations was highlighted using classical molecular biology methods and a PCR amplification assay based on the mmoX gene cluster. Results revealed that significant methanotrophic populations can be maintained in aerobic-anaerobic bioreactors that are initially injected with a pure culture of a methanotrophic bacterium.

DNA probes, Methanobacteriaceae, Methanogens, Bioreactors

---

Bestowing inducibility on the cloned methanol dehydrogenase promoter ([P.sub.mxaF]) of Methylobacterium extorquens by applying regulatory elements of Pseudomonas putida F1

Article Abstract:

Combinatorial methods are adopted to control reporter gene expression at the transcriptional level in Methylobacterium extorquens in order bestow inducibility upon the cloned [P.sub.maxF] promoter in expression vectors. It is the first documented example of an inducible and tightly regulated expression of heterologous genes in Methylobacterium extorquens.

Canada, Genetic transcription, Pseudomonas putida, Transcription (Genetics), Bacterial proteins

---

Multicopy integration and expression of heterologous genes in Methylobacterium extorquens ATCC 55366

Article Abstract:

A study was conducted on the achievement of high-level expression of chromosomally integrated genes in Methylobacterium extorquens ATCC 55366 under the control of the strong extorquens AMI methanol dehydrogenase promoter. The results showed the single and multicopy chromosome integration and stable expression of heterologous genes in M. extorquens.

Bacteria, Heterotrophic, Heterotrophic bacteria, Gene expression, Nucleic acids, Nucleic acid isolation

Similar abstracts:

• Abstracts: Aerobic anoxygeic phototrophic bacteria in the Mid-Atlantic Bight and the North Pacific Gyre. Diversity and abundance of uncultured Cytophaga-like bacteria in the Delaware Estuary
• Abstracts: Influence of environmental conditions on methanogenic compositions in anerobic biogas reactors. Methanosarcina spp. drive vinyl chloride dechlorination via interspecies hydrogen transfer
• Abstracts: Phylogenetic evidence for the existence of novel thermophilic bacteria in hot spring sulfur-turf microbial mats in Japan
• Abstracts: Phylogeographic origin of Hokkaido house mice (Mus musculus) as indicated by genetic markers with maternal, paternal and biparental inheritance
• Abstracts: Diversity of environmental Mycobacterium isolates from hemodialysis water as shown by a multigene sequencing approach

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.