Motility mutants of Vibrio cholerae O1 have reduced adherence in vitro to human small intestinal epithelial cells as demonstrated by ELISA

Article Abstract:

Vibrio cholerae is a Gram-negative pathogenic bacteria which attaches and grows on the surface of the small intestine. To determine the role of cell adhesion in the pathogenesis of cholera, a modified enzyme-linked immunosorbent assay was performed using human small intestinal mucosal tissue. Wild-type V. cholerae strains adhered to epithelial cells. In contrast, motility mutants of V. cholerae which exhibit reduced colonization showed decreased adherence, indicating an association between colonization and the pathogenesis of cholera. Treatment of the epithelial cells with 2-mercaptoethanol inhibited binding of V. cholerae strains, suggesting that adhesion was receptor-specific.

Usage, Vibrio cholerae, Bacterial adhesion, Bacteria, Pathogenic, Pathogenic bacteria, Enzyme-linked immunosorbent assay

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The application of serological techniques to the taxonomy of Arthrobacter and related organisms

Article Abstract:

Previous studies on the genus Arthrobacter have failed to tackle its ecology, due primarily to the lack of methods for identifying and locating these microorganisms in the soil. To resolve this problem, Arthrobacter and Aureobacterium strains produced through clustering techniques are studied using agglutination, immunodiffusion, immunofluorescence and enzyme-linked immunosorbent assay techniques.

Physiological aspects, Microbial ecology

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Growth of Arthrobacter globiformis in soil observed by fluorescent antibody and ELISA techniques

Article Abstract:

ELISA techniques and counting of cell numbers using fluorescent antibodies help evaluate the growth and survival of Arthrobacter globiformis in a sand-dune soil and a wheatfield soil. Growth is observed in both non-sterile and sterile wheatfield soil. Growth of cells directly introduced into soil is faster than the growth of cells introduced on slides. Cells fail to grow in the sand-dune soil.

Analysis, Soil microbiology, Microbial growth

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