New method of denitrification analysis of bradyrhizobium field isolates by gas chromatographic determination of (super 15)N-labeled N(sub 2)

Article Abstract:

A study is conducted to evaluate the denitrification abilities of many bradyhizobium field isolates, for which a new (super 15)N-labeled N(sub 2) detection methodology is developed that is free from interference from atmospheric N(sub 2) contamination. This method enables (super 15)N tracer analysis without the need for isotope ratio determination by mass spectrometry and would overcome some of the problems of the acetylene inhibition method when large number of field isolates is analyzed.

Author: Minamisawa, Kiwamu, Sameshima-Saito, Reiko, Chiba, Kaori
Usage, Risk factors, Microbiology, Gas chromatography

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Expression of 1-aminocyclopropane 1-carboxylic acid deaminase gene requires symbiotic nitrogen-fixing regulator gene nifA2 in Mesorhizobium loti MAFF303099

Article Abstract:

The beta-glucuronidase (GUS) gene was introduced into acdS so that GUS was expressed under control of the acdS promoter and the disruption mutants were generated with mutations in a nitrogen fixation regulator gene, nifA. The main aim behind the effort was to examine the regulation of the acdS gene encoding 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase in Mesorhizobium loti MAFF303099 during symbiosis with the host legume Lotus japonicus.

Author: Nukui, Noriyuki, Minamisawa, Kiwamu, Ayabe, Shin-Ichi, Aoki, Toshio
Analysis, Physiological aspects, Host-bacteria relationships, Gene expression, RNA processing, Lotus (Legumes)

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Rehydration of the lichen Ramalina lacera results in production of reactive oxygen species and nitric oxide and a decrease in antioxidants

Article Abstract:

The effects of rehydration under controlled conditions on ROS-related parameters naturally desiccated Ramalina lacera is studied. Rehydration of R. lacera resulted in the initiation of and a rapid increase in photosynthetic activity.

Author: Weissman, Lior, Garty, Jacob, Hochman, Ayala
Fluid therapy, Photosynthesis

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Subjects list: Research, Rhizobium, Nitric oxide
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