New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library

Article Abstract:

A gene coding for a thermostable esterase was isolated by functional screening of Escherichia coli cells that is transformed with fosmid environmental DNA libraries constructed with metagenomes from thermal environmental samples. The finding of a new thermostable and thermophillic HSL family esterase emphasizes the importance of thermal environmental metagenomic libraries as a source of isolation of novel genes by functional screening in a heterologous E. coli host.

Escherichia coli, Bacteria, Thermophilic, Thermophiles

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Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three- dimensional structure

Article Abstract:

The three-dimensional (3D) structure of the hyperthermophilic esterase EstE1 is constructed by homology modeling using Archaeoglobus fugidus esterase as reference, and the thermostability-structure relationship is analyzed. The results verify the predicted 3D structure of EstE1 and the ion pair networks and hydrophobic interactions that are critical determinants for the thermostability of EstE1 is identified.

Esterases, Hydrophobic effect

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Transgalactosylation in a water-solvent biphasic reaction system with [beta]-galactosidase displayed on the surfaces of Bacillus subtilis spores

Article Abstract:

[beta]-Galactosidase ([beta]-Gal) displayed on Bacillus subtilis spores are used as a whole-cell immobilized biocatalyst for transgalactosylation in water-solvent biphasic reaction systems. The spore display has offered a new way to construct robust biocatalysts along with a novel basis for phase transfer biocatalytic processes.

Biological Product (except Diagnostic) Manufacturing, Drugs, Biological Processors, Biocatalysts, Physiological aspects, Bacillus subtilis, Cell interaction, Cell interactions

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