New threshold and confidence estimates for terminal restriction fragment length polymorphism analysis of complex bacterial communities

Article Abstract:

Different standardizing methods in Terminal restriction fragment length polymorphism ( T-RFLP) analysis of PCR-amplified 16S rRNA genes to elucidate the similarities between the bacterial communities in 17 soil and sediment samples was adopted. With the use of multiple REs, it was possible to determine a confidence estimate for groupings of apparently similar communities and in conjunction with the variable percentage method, conclusions about the similarities of the bacterial communities could be made.

Genetic polymorphisms

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Effect of pH on isolation and distribution of members of subdivision 1 of the phylum acidobacteria occurring in soil

Article Abstract:

A study was conducted to investigate the effects of CO(sub 2) and medium pH on the culturability of subdivision 1 acidobacteria and the phylogenetic diversity of isolates obtained. It was found that moderately acidic pHs favor colony development by members of subdivision 1 of the phylum Acidobacteria and that this group was most abundant in clone libraries prepared from microbial communities from soils around the world with acidic rather than neutral pH.

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