Nickel and cobalt resistance engineered in Escherichia coli by overexpression of serine acetyltransferase from nickel hyperaccumulator plant Thlaspi goesingense
Article Abstract:
The overexpression of serine acetyltransferase from the Ni-hyperaccumulating plant Thlaspi goesingense causes enhanced nickel and cobalt resistance in Escherichia Coli, while the overexpression of Thlaspi goesingense serine acetyltransferase results in enhanced sensitivity to cadmium. Nickel in the serine acetyltransferase-overexpressing strains is not detoxified by coordination or precipitation with sulfur, suggesting that glutathione is involved in reducing the oxidative damage imposed by nickel.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis
Article Abstract:
The construction of newly designed operons for zeaxanthin production by the ordered gene assembly in Bacillus subtilis (OGAB) method which could assemble multiple genes in one step using an intrinsic Bacillus subtilis plasmid transformation system is presented. The results suggest that rearrangement of biosynthetic genes in the order of the metabolic pathway by the OGAB method could be a useful approach for metabolic engineering.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Cloning, sequencing and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6
- Abstracts: Phylogeny and strain typing of Escherichia coli, inferred from variation at mononucleotide repeat loci. Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses
- Abstracts: Design and application of highly responsive fluorescence resonance energy transfer biosensors for detection of sugar in living Saccharomyces cerevisiae cells
- Abstracts: Microbial quality and direct PCR identification of lactic acid bacteria and nonpathogenic staphylococci from artisanal low-acid sausages. part 2
- Abstracts: Effects of mutations in the substrate-binding domain of poly[(R)-3-hydroxybutyrate] (PHB) depolymerase from Ralstonia pickettii T1 on PHB degradation