No end yet to messenger RNA 3' processing

Article Abstract:

In mammals, the messenger RNA 3' end-processing biochemical reaction involves endonucleolytic cleavage and addition of a part of the poly(A)polymerase. Substrate recognition is controlled by the cleavage and polyadenylation specificity factor (CPSF) and the cleavage stimulation factor (CstF). In yeast the 3' end-processing reaction and the structure of the poly(A)polymerase is similar to that of mammals and even though the function of CstF and the yeast CF1 are not the same their structure is similar. The yeast CF1 is functionally similar to mammalian CPSF.

Messenger RNA

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Contact with a component of the polymerase II holoenzyme suffices for gene activation

Article Abstract:

Gene activation is initiated by the interaction of holoenzyme with an activator by sending the former to DNA. A point mutation in yeast turns the holoenzyme into a new interaction site for a peptide with no activator. A fusion of DNA-bound protein with a single holoenzyme activator initiates gene activation by sending holoenzyme to the former. Experiments show that such interactions trigger off strong gene activation with the help of transcriptional activators such as GAL 11 and GAL 4.

Microbial mutation

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The FIP1 gene encodes a component of the yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase

Article Abstract:

The gene, FIP1 encodes a protein which interacts with the poly(A) polymerase (PAP1) enzyme of yeast in a two-hybrid system. fip1 mutants are able to cleave the primary transcripts but cannot polyadenylate the cleaved products. The polyadenylation property is restored by the addition of the polyadenylation factor (PF I). The PF I binds the PAP1 to the cleavage factor which makes PAP1 specific to pre-mRNA substrates.

Genetic aspects, Yeast, Yeast (Food product)

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