Novel assay for screening rifamycin B-producing mutants

Article Abstract:

A simple procedure for screening rifamycin B-producing organisms was described. The test involved using rifamycin oxidase, an enzyme that converts inactive rifamycin B to active rifamycin S. In this assay, colonies to be identified were grown in pairs and transferred to two plates seeded with Staphylococcus aureus where one plate contains rifamycin oxidase. Paired colonies with a larger inhibition zone diameter on the plate containing the enzymes were identified as rifamycin-producing strains.

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A simple, high-precision, high-sensitivity tracer assay for N2 fixation

Article Abstract:

An experimental protocol capitalizing on high analytical sensitivity to directly measure N2 fixation has been developed. The protocol was applied by a high-precision isotope ratio mass spectrometer. Fixation was measured in planktonic organisms inhabiting the central Baltic Sea. The 15N2 protocols developed are comparable to standard C2H2 procedures. The 15N tracer method has the potential to directly track fixed N movement into other biologically active pools.

Biology, Nitrogen, Nitrogen fixation, Radioactive tracers, Radioactive tracers in biology

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The spheroplast lysis assay for yeast in microtiter plate format

Article Abstract:

Researchers have succeeded in adapting the yeast spheroplast lysis assay to a microtiter format. Using this format, optimum cell number and enzyme use were decreased 15-fold. The assay could be used to screen bacterial strains for their susceptibility to specific drugs.

Research, Physiological aspects, Drug resistance in microorganisms, Microbial drug resistance, Yeast, Yeast (Food product), Spheroplasts

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