Novel assay for screening rifamycin B-producing mutants
Article Abstract:
A simple procedure for screening rifamycin B-producing organisms was described. The test involved using rifamycin oxidase, an enzyme that converts inactive rifamycin B to active rifamycin S. In this assay, colonies to be identified were grown in pairs and transferred to two plates seeded with Staphylococcus aureus where one plate contains rifamycin oxidase. Paired colonies with a larger inhibition zone diameter on the plate containing the enzymes were identified as rifamycin-producing strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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A simple, high-precision, high-sensitivity tracer assay for N2 fixation
Article Abstract:
An experimental protocol capitalizing on high analytical sensitivity to directly measure N2 fixation has been developed. The protocol was applied by a high-precision isotope ratio mass spectrometer. Fixation was measured in planktonic organisms inhabiting the central Baltic Sea. The 15N2 protocols developed are comparable to standard C2H2 procedures. The 15N tracer method has the potential to directly track fixed N movement into other biologically active pools.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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The spheroplast lysis assay for yeast in microtiter plate format
Article Abstract:
Researchers have succeeded in adapting the yeast spheroplast lysis assay to a microtiter format. Using this format, optimum cell number and enzyme use were decreased 15-fold. The assay could be used to screen bacterial strains for their susceptibility to specific drugs.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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- Abstracts: The Saccharomyces cerevisiae Ku autoantigen homologue affects radiosensitivity only in the absence of homologous recombination
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