Novel methods for genetic transformation of natural Bacillus subtilis isolates used to study the regulation of the mycosubtilin and surfactin synthetases

Article Abstract:

Two novel methods that stimulate the natural and genetic transformation of natural isolates of Bacillus subtilis, which has low genetic competence levels, are described. The methods can be easily used to study the regulation of synthetases of mycosubtilin and surfactin, the two commonly used lipopeptide antibiotics.

Analysis, Genetic transformation

---

Changing a single amino acid in Clostridium perfringens [beta]-toxin affects the efficiency of heterologous secretion by Bacillus subtilis

Article Abstract:

The studies related to differences in the global gene regulation responses of Bacillus subtilis to overproduction of either [beta]-toxin or [beta]-toxoid by transcriptomics showed that the intrinsic properties of a heterologous protein could play a decisive role when optimizing heterologous protein production.

Bacterial proteins

---

Expression of transcription activator comK of Bacillus subtilis in the heterologous host Lactococcus lactis leads to a genome-wide repression pattern: A case study of horizontal gene transfer

Article Abstract:

A study was conducted on horizontal gene transfer (HGT) by introduction of the competence transcription factor comK of B. subtilis into the recipient L. lactis. It was observed that comK is not functional in L. lactis as it is in B. subtilis.

Microbial genetics, Lactococcus

Similar abstracts:

• Abstracts: Factors influencing germination of Bacillus subtilis spores via activation of nutrient receptors by high pressure
• Abstracts: Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: Strict control of gene expression by addition of subtilin
• Abstracts: Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome
• Abstracts: Genetic modification of Bacillus subtilis for production of D-chiro-inositol, an investigational drug candidate for treatment of type 2 diabetes and polycystic ovary syndrome
• Abstracts: Use of a disposable water filter for prevention of false-positive results due to nontuberculosis mycobacteria in a clinical laboratory performing routine acid-fast staining for tuberculosis

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.