Determination of virus abundance in marine sediments
Article Abstract:
Researchers optimized the measurement of viruses in marine sediment samples using pyrophosphate as the surfactant and three minutes of ultrasound. SYBR Green I was used as a stain, followed by epifluorescence microscopy.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Comparison of two engineering techniques, terminal restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis, for determination of bacterial diversity in aquatic environments
Article Abstract:
Bacterial diversity in different aquatic environments is investigated and two fingerprinting techniques (terminal restriction fragment length polymorphism [T-RFLP] and automated ribosomal intergenic spacer analysis [ARISA]), which are currently utilized for estimating richness and community composition, are compared. Results reveal that ARISA is more accurate than T-RFLP analysis on the 16S rRNA gene for estimating the biodiversity of aquatic bacterial assemblages.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Nucleic acid (DNA, RNA) quantification and RNA/DNA ratio determination in marine sediments: comparison of spectrophotometric, fluorometric, and high-performance liquid chromatography methods and estimation of detrital DNA
Article Abstract:
Research was conducted to examine the nucleic acid (DNA, RNA) quantification and RNA/DNA ratio determination in marine sediments. A comparison of the extraction and quantification methods, namely, spectrophotometric, high-pressure liquid chromatography and fluorometric, which differed in relation to the nucleic acid extraction procedure, was performed. Based on fluorometric estimates, it is shown that the RNA/DNA ratio can be useful as an indicator of benthic metabolic activity only when metazoan contribution to the microbial DNA is very minimal.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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