Determination of virus abundance in marine sediments

Article Abstract:

Researchers optimized the measurement of viruses in marine sediment samples using pyrophosphate as the surfactant and three minutes of ultrasound. SYBR Green I was used as a stain, followed by epifluorescence microscopy.

Viruses, Marine microbiology

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Comparison of two engineering techniques, terminal restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis, for determination of bacterial diversity in aquatic environments

Article Abstract:

Bacterial diversity in different aquatic environments is investigated and two fingerprinting techniques (terminal restriction fragment length polymorphism [T-RFLP] and automated ribosomal intergenic spacer analysis [ARISA]), which are currently utilized for estimating richness and community composition, are compared. Results reveal that ARISA is more accurate than T-RFLP analysis on the 16S rRNA gene for estimating the biodiversity of aquatic bacterial assemblages.

Italy, Science & research, Water, Aquatic microbiology, Biological diversity, Biodiversity, Microbial ecology

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Nucleic acid (DNA, RNA) quantification and RNA/DNA ratio determination in marine sediments: comparison of spectrophotometric, fluorometric, and high-performance liquid chromatography methods and estimation of detrital DNA

Article Abstract:

Research was conducted to examine the nucleic acid (DNA, RNA) quantification and RNA/DNA ratio determination in marine sediments. A comparison of the extraction and quantification methods, namely, spectrophotometric, high-pressure liquid chromatography and fluorometric, which differed in relation to the nucleic acid extraction procedure, was performed. Based on fluorometric estimates, it is shown that the RNA/DNA ratio can be useful as an indicator of benthic metabolic activity only when metazoan contribution to the microbial DNA is very minimal.

Analysis, Marine sediments, Spectrophotometry, Nucleic acids, Bacteriology, Fluorimetry, High performance liquid chromatography

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