Nucleotide sequence of the Bacillus subtilis dnaD gene

Article Abstract:

Cloning of a 104 kb DNA segment of the trp operon-containing Bacillus subtilis chromosome in an artificial, yeast chromosome reveals the presence of the dnaD gene. Nucleotide sequencing reveals that dnaD codes for a protein of 232 amino acids. A putative ribosome-binding site (RBS) is located 7 bp upstream of the start codon, while a putative p-independent transcription terminator is found nearly 30 bp upstream of the RBS. Two open-reading frames that can be cotranscribed with dnaD are present downstream of dnaD.

Nucleotide sequence, Base sequence

---

The Bacillus subtilis dnal gene is part of the dnaB operon

Article Abstract:

The genes regulating DNA replication in Bacillus subtilis in various environmental conditions have been identified. A mutation of the dnaI gene, dnaI2, was thought to be a controller of replication when the organism experiences non-viable temperatures. The gene has been located and mapped on the B. subtilis chromosome to show that the mutation involves substitutions on the nucleotide sequence. The wild type gene was found to be part of a sequence that also codes for dnaB.

Research, Bacteria, Genetic transcription, Operons, Transcription (Genetics)

---

Mapping of the 150kb spoIIIC-pheA region of the Bacillus subtilis chromosome using Long Accurate PCR and three yeast artificial chromosomes

Article Abstract:

The 150kb spoIIIC-pheA region of Bacillus subtilis chromosome was cloned and sequenced by Long Accurate (LA) polymerase chain reaction (PCR). Amplification of the sequenced genes involves the generation of eight random sequence tags from yeast artificial chromosome PFGE-purified deoxyribonucleic acid (DNA). The chromosome mapping technique efficiently generated sufficient DNA segments for the sequencing of Bacillus subtilis 150kb spoIIIC-pheA region.

Methods, Polymerase chain reaction, Genomes, Chromosome mapping, Yeast, Yeast (Food product), Genetic code

Similar abstracts:

• Abstracts: Systematic sequencing of the Bacillus subtilis genome: progress report of the Japanese group. Determination of a 17484 bp nucleotide sequence around the 39 degree region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs
• Abstracts: The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes
• Abstracts: The Bacillus subtilis chromosome region encoding homologues of the Escherichia coli mssA and rpsA gene products

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.