Okadaic acid induces spindle lengthening and disrupts the interaction of microtubules with the kinetochores in metaphase II-arrested mouse oocytes

Article Abstract:

Oocytes were incubated with okadaic acid (OA) to determine whether the meiotic spindle proteins of metaphase II-arrested mouse oocytes are substrates for phosphorylation events. Results revealed that OA incubation caused the spindles to significantly increase in length and disrupted the interactions between microtubules and kinetochores, resulting in a disorganized metaphase plate. Alterations were also detected in the phosphorylation pattern. These indicate that the phosphorylation of certain proteins associated with the microtubules are partly responsible for changes taking place in the microtubule network during the cell cycle.

Phosphatases, Spindle (Cell division), Spindle (Cytoplasm), Enzyme inhibitors, Oogenesis

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Calmodulin-dependent protein kinase II is activated transiently in ethanol-stimulated mouse oocytes

Article Abstract:

A study of the activity of calmodulin-dependent protein kinase II (CaMKII) in mouse oocytes arrested in metaphase II, after parthenogenic activation, showed that CaMKII activation occurs in response to an increase in intracellular calcium and acts upstream of the microtubule-dependent cyclin destruction mechanism. The kinase becomes highly active after the oocytes are exposed to ethanol. Moreover, nocodazole, a microtubule-depolymerizing drug that blocks the exit from M phase in mouse oocytes, does not affect CaMKII activation.

Analysis, Xenopus, Protein kinases

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Serotonin-induced meiosis reinitiation from the first prophase and from the first metaphase in oocytes of the marine bivalve Hiatella flaccida: respective changes in intracellular Ca2+ and pH

Article Abstract:

The amplitude and pattern of a serotonin-induced intracellular Ca2+ alterations are discrete for prophase-I and metaphase-I oocytes of Hiatella flaccida. The serotonin, 5-HT, is responsible for reinitiating meiosis when the oocytes are stalled at these stages. 5-HT causes a rise in intracellular Ca2+ in metaphase-I oocytes but increases both intracellular Ca2+ and pH in prophase-I oocytes. Meiosis reinitiation from prophase-I is not responsible for the rise in intracellular pH.

Calcium ions, Hydrogen-ion concentration, Serotonin

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