Abstracts » Biological sciences

Physiological properties of a Pseudomonas strain which grows with p-xylene in a two-phase (organic-aqueous) medium

Article Abstract:

Pseudomonas putida strain Idaho, which has the ability to grow on concentrated solutions of p-xylene, toluene and other alkylbenzenes as sole carbon and energy sources, was characterized physiologically. The results showed that the bacterium's ability to grow on organic solvents was correlated with the logarithm of the octanol-water partition coefficient. Morphological changes during growth included the convolution and shedding of the outer membrane, and the invagination and vesicle-formation of the cytoplasmic membrane. Hybridization experiments showed the presence of sequences homologous to the upper operon in the TOL plasmid pWWO.

Aromatic compounds, Xylene, Xylenes

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Regio- and stereospecific oxidation of 9,10-dihydroanthracene and 9,10-dihydrophenanthrene by naphthalene dioxygenase: structure and absolute stereochemistry of metabolites

Article Abstract:

Naphthalene dioxygenase (NDO), expressed by Pseudomonas putida, catalyzes regio- and stereo-specific dihydroxylation of 9,10-dihydroanthracene (DHA) and 9,10-dihydrophenanthrene (DHP). The dihydroxylation produces previously undescribed homochiral cis-diene diols of R configuration at the bridgehead carbon atom-adjacent benzylic center. The location of the benzylic carbons affects the type of reaction that NDO catalyzes in benzocyclic substrates. The regio- and stereo-chemistry of the DHP and DHA cis-diols is consistent with the cis-diols of naphthalene, anthracene and phenanthrene.

Research, Oxidation, Physiological, Physiological oxidation, Naphthalene, Hydrocarbons

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The alpha subunit of toluene dioxygenase from Pseudomonas putida F1 can accept electrons from reduced ferredoxin(sub TOL) but is catalytically inactive in the absence of the beta subunit

Article Abstract:

A high-expression clone for todC1 has been created while the purification and properties of TodC1 have been studied. The electron transfer between Ferredoxin(sub TOL) and TodC1 has been examined while TDO activity from purified TodC1 and C2 subunits have been reconstituted in vitro. TodC1 and C2 are the alpha and beta subunits of the oxygenase component of toluene dioxygenase from Pseudomonas putida F1. Reductase(sub TOL) is a flavoprotein reductase that transfers electrons from NADH to the Rieske protein, Ferrodoxin(sub TOL).

Enzymes, Oxidases, Enzyme structure-activity relationships

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