Plant genome complexity may be a factor limiting in situ the transfer of transgenic plant genes to the phytopathogen Ralstonia solanacearum

Article Abstract:

Research demonstrates that effective transfer of eukaryotic genes to prokaryotic systems is influenced by the ratio of eukaryotic genome complexity and the number copies of the transgene as shown in a study involving transfer of plant genes into Ralstonia solanacearum bacteria.

United States, France, Statistical Data Included, Analysis, Genetic aspects, Genetically modified plants, Eukaryotic cells, Prokaryotes, Cells (Biology), Eukaryotes, Bacterial genetics, Genomes

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Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction

Article Abstract:

A protocol for the detection and enumeration of bacteria in soil was developed using polymerase chain reaction techniques. The procedure involved the use of successive steps of sonication, microwave heating and thermal shocks to maximize the DNA yields. Subsequent purification steps involved the use of Elutip d columns in three passages. Tests on artificially-inoculated soil samples showed the validity of the procedure. An actual application was perfomed to detect and estimate the indigenous populations of Frankia spp.

Methods, Usage, Measurement, Soil microbiology, Polymerase chain reaction

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Conditions for natural transformation of Ralstonia solanacearum

Article Abstract:

A research was conducted to study the in vitro transformation conditions and parameters for the development of Ralstonia solanacearum. The transformation efficiencies were determined with various concentrations of genomic DNA extracted from strain GMI1004. Results suggested that the lack of transformants with foreign linear DNA was probably not due to a DNA restriction system. There were also similarities in the transformation process to that of the human pathogen N. gonorrhoeae.

Bacterial transformation

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