Production of a polyhydroxyalkanoate biopolymer in insect cells with a modified eucaryotic fatty acid synthase

Article Abstract:

A method for the synthesis of polyhydroxybutyrate (PHB) in cells of the insect Spodoptera frugiperda involves the activity of a rat mutant fatty acid synthase (FAS) and the Alcaligenes eutrophus phbC gene encoding polyhydroxyalkanoate synthase. The FAS has a mutation in the dehydrase region and catalyzes the production of R(-)-3-hydroxybutyryl-coenzyme A as the premature termination product. The coenzyme is converted into PHB in the insect cells. The method can be used in both prokaryotic and eukaryotic cells and provides a means of synthesizing novel polyhydroxyalkanoate bioplastics.

Microbial enzymes, Biopolymers, Polyhydroxybutyrate

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Phylogenetic ecology of the freshwater Actinobacteria acI lineage

Article Abstract:

The acI 16S rRNA genes and 16S-rRNA internal transcribed spacer regions from Wisconsin lakes are studied and standard nonphylogenetic and phylogenetic statistical approaches are used for examining the factors that have determined acI community composition at the local scale and at the regional scale. The nonrandom distribution of these acI clades has indicated potential ecophysiological differences between the clades, with acI clades AI, BII and BIII preferring acidic lakes and acI clades AII, AVI and BI preferring more alkaline lakes.

Genetic aspects, Cladistic analysis

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Isolation and characterization of novel marine-derived actinomycete taxa rich in bioactive metabolites

Article Abstract:

A unique selective enrichment procedure that resulted in the isolation and identification of two novel marine-derived actinomycete taxa from the Bismarck Sea and the Solomon Sea is presented. A combination of physiological parameters, chemotaxonomic characteristics and phylogenetic analysis based on 16S rRNA genes provided strong evidence for the two new genera.

Isolating mechanisms, Properties, Metabolites, Reproductive isolating mechanisms

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