Promoter elements in Drosophila melanogaster revealed by sequence analysis

Article Abstract:

Analysis of Drosophila promoter database of 252 independent promoter entries reveals the presence of significant nucleotide sequence irregularities in the proximal promoter region. This region has a tripartite structure, with initiator at plus or minus 5 bp, TATA at -25/-30 bp and a new group of downstream elements at +20/+30 bp from the RNA start site. The strand-specific downstream elements vary from the initiator and TATA in their length, position, representation and multiple sequences. An extended region with increased GC- and decreased A-content with several binding sites for Drosophila transcription factors constitute the overall sequence organization of the promoter region.

Promoters (Genetics)

---

A short 5'-flanking region mediates glucose repression of amylase gene expression in Drosophila melanogaster

Article Abstract:

Glucose repression of amylase gene expression was studied in Drosophila melanogaster. The Bio-Rad protein assay was used to determine amylase expression. Results showed that cis-acting elements in the amylase promoter caused sufficient glucose-repressible gene expression while ADH repression was variable in glucose and non-glucose fed larvae. Proximal and distal amylase promoters were compared and sequence divergence of as much as 40% was noted. Depression of the amylase gene was controlled by upstream promoters.

Enzymes, Enzyme regulation

---

Genetic analysis of the Additional sex combs locus of Drosophila melanogaster

Article Abstract:

Gamma radiation was used to isolate new alleles of the Additional sex combs (Asx) locus of Drosophila melanogaster. The Asx region had a higher mutation rate than expected, hence it is either sensitive to radiation damage or is a large gene. Loss of the Asx gene resulted in segmentation defects and altered patterns of expression of the even-skipped gene, which suggests that Asx is a developmental requisite. Transposon tagging of the Asx locus was performed, allowing molecular studies to be started.

Similar abstracts:

• Abstracts: P elements and P-M characteristics in natural populations of Drosophila melanogaster in the southernmost islands of Japan and in Taiwan
• Abstracts: Potential early intermediates in anaerobic benzoate degradation by Rhodopseudomonas palustris. Reductive, coenzyme A-mediated pathway for 3-chlorobenzoate degradation in the phototrophic bacterium Rhodopseudomonas palustris
• Abstracts: Genomic distribution of P elements in Drosophila willistoni and a search for their relationship with chromosomal inversions
• Abstracts: Evidence of a dual function in fl(2)d, a gene needed for sex-lethal expression in Drosophila melanogaster. Differentiation of a male-specific muscle in Drosophila melanogaster does not require the sex-determining genes doublesex or intersex
• Abstracts: Gene centromere mapping of six enzyme loci in gynogenetic channel catfish. Inheritance and variation of genome size in half-sib families of hybrid catfishes

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.