Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3- hydroxyalkanoates) consisting of saturated and unsaturated monomers
Article Abstract:
Pseudomonas putida KT2442 is a strain that can make use of simple carbohydrate substrates such as glucose, fructose and glycerol as its carbon and energy sources. Poly(3-hydroxyalkanoates) or PHAs are usually accumulated by bacteria as their carbon and energy reserves utilizing related substrates for PHA synthesis. The related substrates usually used are fatty acids. This study proves that PHA synthesis is possible using nonrelated substrates. These substrates enable PHA formation subject to cultivation temperature. Saturated and unsaturated monomers of PHA form as intermediate products of de novo fatty acid biosynthesis with the degree of unsaturation depending on the temperature. PHA is synthesized from a common intermediate product in the carbohydrate substrate metabolism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Reduction of cell lysate viscosity during processing of poly(3-hydroxyalkanoates) by chromosomal integration of the staphylococcal nuclease gene in Pseudomonas putida
Article Abstract:
The staphylococcal nuclease gene in Pseudomonas putida was integrated into the chromosomes of different poly(3-hydroxyalkanoate) (PHA) producers to derive a commercially viable solution to the viscosity problem in PHA processing. A rapid increase in the viscosity of the cell lysate which hampers filtration and centrifugation of PHAs as a new resource for plastic materials. Staphylococcal nuclease is easily expressed in Pseudomonas strains that produce PHA and is directed to the periplasm without diminishing PHA production or strain stability.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Accumulation of poly[(R)-3-hydroxyalkanoates] in Pseudomonas oleovorans durign growth with octanoate in continuous culture at different dilution rates
Article Abstract:
Pseudomonas oleovorans has three growth stages. One occurs at low carbon concentrations, one at low nitrogen concentrations, and one when both elements are limited. It accumulates poly[(R)-3-hydroxyalkanoates during nitrogen limitation and carbon-nitrogen limitation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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