Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus
Article Abstract:
The pyrE and pyrF genes that are needed for pyrimidine synthesis have been isolated from uracil auxotrophic mutants of the thermophile Thermus thermophilus. The pyrE gene encodes orotate phosphoribosyltransferase (OPRTase) and is similar to the OPRTase genes of other microorganisms. Its nucleotide and amino acid sequences are 98.5% and 100% identical to those of the T. flavus pyrE. The pyrF gene encodes orotidine-5'-monophosphate decarboxylase and has a conserved sequence. The pyrE-pyrF sequence can be used to integrate and express foreign genes such as the leucine operon.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Isocitrate dehydrogenase from Thermus aquaticus YT1: purification of the enzyme and cloning, sequencing, and expression of the gene
Article Abstract:
Isocitrate dehydrogenase from Thermus aquaticus YT1 harbors a single open reading frame of 1,278 bp, which is preceded by a Shine-Dalgarno ribosome binding site. A terminator-like sequence is located downstream of the open reading frame. The amino acid sequence of this enzyme is highly similar to that of its counterparts from Escherichia coli and Bacillus subtilis, but shares little similarity with the counterpart from T. thermophilus. The cloned gene of isocitrate dehydrogenase is highly expressed in Escherichia coli, and can be purified to homogeneity easily.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermopile, Thermus thermophilus HB8
Article Abstract:
The genetic code for isocitrate dehydrogenase of Thermus thermophilus HB8 was determined by molecular cloning and DNA sequencing. The gene was composed of open reading frame with promoter- and terminator like sequences. The guanine + cytosine composition of the coding region was determined as 65.%. The molecular weight of the monomeric unit was calculated as 54,189. Comparative characterization with thatof the Escheruchia coli was also performed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp
- Abstracts: Glutamine synthetase and nitrogen cycling in colonies of the marine diazotrophic cyanobacteria Trichodesmium spp
- Abstracts: Purification and characterization of an extremely thermostable cyclomaltodextrin glucanotransferase from a newly isolated hyperthermophilic archaeon, a Thermococcus sp
- Abstracts: Molybdenum oxidation by Thiobacillus ferrooxidans. Isolation and characterization of a Mo6+-reducing bacterium
- Abstracts: Meiotic nondisjunction and recombination of chromosome III and homologous fragments in Saccharomyces cerevisiae