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Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones

Article Abstract:

A study was conducted on the gram-negative bacterium, Chromobacterium violaceum, and quorum sensing. The findings indicate that this bacteria produces N-acylhomoserine lactones, which serve as signal molecules for quorum sensing. Moreover, N-hexonoyl-L-homoserine lactone plays a role in the regulation of exoprotease, chitinase and cyanide production. Thus, stimulation or inhibition of violacein production in a violacein-negative, mini-Tn5 mutant of C violaceum can be used in plate bioassays and thin-layer chromatography overlays.

Author: Williams, Paul, Taylor, Adrian, Stewart, Gordon S.A.B., McClean, Kay H., Winson, Michael K., Fish, Leigh, Chhabra, Siri Ram, Camara, Miguel, Daykin, Mavis, Lamb, John H., Swift, Simon, Bycroft, Barrie W.
Publisher: Society for General Microbiology
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
Gram-negative bacteria, Lactones, Biological assay, Bioassay

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Challenging food microbiology from a molecular perspective

Article Abstract:

The functions and contributions of bacterial bioluminescence in food microbiology were cited. Known as the 'living light', bioluminescence explains the relationship between metabolism and light emission. It was cited as a significant tool which enable researchers to consider the persistent problems within food microbiology such as bacterial detection and control.

Author: Stewart, Gordon S.A.B.
Publisher: Society for General Microbiology
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
Usage, Bacteriophages, Bioluminescence, Molecular microbiology

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