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Rapid and sensitive method for detection of shiga-like toxin-producing Escherichia coli in ground beef using the polymerase reaction

Article Abstract:

The amplification of Shiga-like toxins gene targets using two sets of oligonucleotide primer pairs is discussed. Ground beef samples were innoculated with the apppropriate toxin strains and cultured in modified trypticase soy broth for six hours at 42 degrees centigrade. DNA extraacted from the broth cultures showed that PCR assays can detect an initial sample inocolum of as few as one CFU.

Author: Gannon , Victor P.J., King, Robin K., Kim, Jong Y., Thomas, Elizabeth J. Golsteyn
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
Analysis, Escherichia coli

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Twelve-hour PCR-based method for detection of Salmonella spp. in food

Article Abstract:

Researchers describe a PCR technique that can detect Salmonella in food samples in 12 hours or less. Culture-based identification takes five or six days, during which time the food cannot be sold.

Author: Ferretti, R., Mannazzu, I., Cocolin, L., Comi, G., Clementi, F.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
Measurement, Food contamination

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Rapid approach to determine rrn arrangement in Salmonella serovars

Article Abstract:

Researchers have designed PCR primers for the regions surrounding the seven rrn operons of Salmonella enterica serovars. The rrn operons are the source of chromosomal rearrangements in bacteria.

Author: Helm, R. Allen, Maloy, Stanley
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
Research, Genetic aspects, Translocation (Genetics)

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Subjects list: Usage, Polymerase chain reaction, Salmonella
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