Rapid detection, identification, and enumeration of Escherichia coli cells in municipal water by chemiluminescent in situ hybridization

Article Abstract:

A new, one-day method is presented for chemiluminescent in situ hybridization for detecting, identifying and enumerating Escherichia coli cells in municipal water supplies. The method uses soybean peroxidase-labeled peptide nucleic acid (PNA) probes with a 47-mm diameter membrane filter, providing additional specificity and sensitivity while allowing the use of conventional culturing and filtration techniques.

United States, Statistical Data Included, Methods, Management, Usage, Escherichia coli, Water, In situ hybridization, Identification and classification, Water treatment, Water quality management, Peroxidase, Municipal water supply, Chemiluminescence

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Molecular detection and identification of Brettanomyces/Dekkera bruxellensis and Brettanomyces/Dekkera anomalus in spoiled wines

Article Abstract:

The development of a PCR-restriction enzyme (RE) analysis protocol to detect and identify, B. bruxellensis and B. anomalus, is described. Other molecular techniques, such as PCR-DGGE, reverse transcription (RT)-PCR-DGGE, and hybridization with a B.bruxellensis-specific probe, are used as confirmatory tests for the detection of spoilage yeasts in wine samples.

Science & research, Genetic aspects, Genetic transcription, Transcription (Genetics), Genetic research

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Identification of Dekkera bruxellensis (Brettanomyces) from wine by fluorescence in situ hybridization using peptide nucleic acid probes

Article Abstract:

Researchers describe a peptide nucleic acid probe for the 26S ribosomal DNA of Dekkera bruxellensis that can identify this yeast with 100% sensitivity. This organism is responsible for wine spoilage and is known in the wine industry as Brettanomyces.

Industrial microorganisms

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