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Abstracts » Biological sciences

Rapid detection, identification, and enumeration of Escherichia coli cells in municipal water by chemiluminescent in situ hybridization

Article Abstract:

A new, one-day method is presented for chemiluminescent in situ hybridization for detecting, identifying and enumerating Escherichia coli cells in municipal water supplies. The method uses soybean peroxidase-labeled peptide nucleic acid (PNA) probes with a 47-mm diameter membrane filter, providing additional specificity and sensitivity while allowing the use of conventional culturing and filtration techniques.

Author: Stender, Henrik, Broomer, Adam J., Oliveira, Kenneth, Perry-O'Keefe, Heather, Hyldig-Nielsen, Jens J., Sage, Andrew, Coull, James
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
United States, Statistical Data Included, Methods, Management, Usage, Escherichia coli, Water, In situ hybridization, Identification and classification, Water treatment, Water quality management, Peroxidase, Municipal water supply, Chemiluminescence

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Molecular detection and identification of Brettanomyces/Dekkera bruxellensis and Brettanomyces/Dekkera anomalus in spoiled wines

Article Abstract:

The development of a PCR-restriction enzyme (RE) analysis protocol to detect and identify, B. bruxellensis and B. anomalus, is described. Other molecular techniques, such as PCR-DGGE, reverse transcription (RT)-PCR-DGGE, and hybridization with a B.bruxellensis-specific probe, are used as confirmatory tests for the detection of spoilage yeasts in wine samples.

Author: Cocolin, Luca, Comi, Giuseppe, Rantsiou, Kalliopi, Iacumin, Lucilla, Zironi, Roberta
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Science & research, Genetic aspects, Genetic transcription, Transcription (Genetics), Genetic research

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Identification of Dekkera bruxellensis (Brettanomyces) from wine by fluorescence in situ hybridization using peptide nucleic acid probes

Article Abstract:

Researchers describe a peptide nucleic acid probe for the 26S ribosomal DNA of Dekkera bruxellensis that can identify this yeast with 100% sensitivity. This organism is responsible for wine spoilage and is known in the wine industry as Brettanomyces.

Author: Oliveira, Kenneth, Perry-O'Keefe, Heather, Hyldig-Nielsen, Jens J., Sage, Andrew, Coull, James, Stender, Hendrik, Kurtzman, Cletus, Sorensen, Ditte, Broomer, Adam, Young, Barbara
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
Industrial microorganisms

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Subjects list: Research, Yeast fungi, Yeasts (Fungi)
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