Regulation of the phosphate stress response in Rhizobium meliloti by PhoB

Article Abstract:

A research was conducted to study the responses of Rhizobium meliloti to phosphate stress and to determine the phosphate level at which R. meliloti induces its P stress response. Transposon Tn5 was conjugated from Escherichia coli S17-1 into R. meliloti via the suicide vector pSUP2021. Alfalfa seedlings in sterile growth box units were utilized to determine the symbiosis phenotype of the phoB mutant. Results indicated that the loss of a functional PhoB did not disturb the symbiotic function of R. meliloti regardless of the available phosphate.

Phosphates

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New recombination methods for sinorhizobium meliloti genetics

Article Abstract:

The availability of bacterial genome sequences has created a need for improved method for sequence-based funtionalized analysis to facilitate moving from annotated DNA sequence to genetic for analyzing the roles that postulated genes play in bacterial phenotypes. A powerful cloning method that uses lambda integrase recombination to clone and manipulate DNA sequences is adapted for use with the gram-negative alpha-proteobacterium sinorhizobium meliloti in two ways.

Nucleotide sequence, Base sequence, Bacteriology

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Development of a functional genomics platform for Sinorhizobium meliloti: Construction of an ORFeome

Article Abstract:

A strategy for genetic analysis of Sinorhizobium meliloti on a genomic scale is presented and the successful implementation of the first step of this strategy is reported. The strategy could be generalized to many other genome projects, and the Sinorhizobium meliloti clones should be useful for investigators wanting an accessible source of cloned genes encoding specific enzymes.

Genetic aspects, Genetic code

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