Relationship between Legionella pneumophila and Acanthamoeba polyphaga: physiological status and susceptibility to chemical inactivation

Article Abstract:

The susceptibility of Legionella pneumophila grown under iron-depleted and iron-sufficient conditions to three biocides, polyhexametheylene biguanide (PHMB), benzisothiazolone (BIT) and 5-chloro-n-methylisothiazolone (CMIT), was studied. BIT was relatively ineffective against iron-depleted cells, while the efficiency of CMIT and PHMB increased. The activity of all three biocides was reduced for Legionella grown intracellularly in Acanthamoeba polyphaga, but PHMB proved to be the most effective antimicrobial. Few amoebae likewise survived CMIT treatment, but BIT was found ineffective.

Biocides, Legionella pneumophila

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Infection of Acanthamoeba polyphaga with Simkania negevensis and S. negevensis survival within amoebal cysts

Article Abstract:

Protozoa of the genus Acanthamoeba may act as reservoirs for Simkania negevensis, a recently discovered chlamydia-like microorganism. This microorganism can cause respiratory disease in humans. The organism infects the amoeba and can survive in the amoebal cysts for long periods of time.

Chlamydia

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Evidence for the presence of Simkania negevensis in drinking and in reclaimed wastewater in Israel

Article Abstract:

Evidence of the presence of Simkania, an intracellular bacterium, in drinking water as well as in reclaimed wastewater is seen in the study. This detection is made possible by the development of a filter membrane immunoassay in the water samples.

Science & research, Growth, Cell membranes, Drinking water, Immunoassay, Company growth

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