Role of tryptophan residues in toxicity of Cry1Ab toxin from Bacillus thuringiensis

Article Abstract:

A study was conducted to examine the role of Trp residues in the structure and function of Cry1Ab toxin, produced by Bacillus thuringiensis. The results show that W65 and W316 are important for insecticidal activity of the toxin since their replacement by Phe reduced toxicity against Manduca sexta.

Author: Sanchez, Jorge, Dean, Donald H., Alzate, Oscar, Riva, Gustavo de la, Padilla, Christopher, Pardo-Lopez, Liliana, Gomez, Isabel, Hernandez, Georgina, Nunez, Maria Eugenia, Carey, Marianne P., Soberon, Mario
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Science & research, Genetic aspects, Bacillus thuringiensis, Diseases and pests, Tryptophan, Antibiotic residues

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Production of chymotrypsin-resistant Bacillus thuringiensis Cry2Aa1 delta-endotoxin by protein engineering

Article Abstract:

Research was conducted to produce mutant forms of Cry2Aa1 delta-endotoxin of Bacillus thuringiensis using site-directed mutagenesis. Results show that midgut enzymes of gypsy moth on these mutant forms produce the active fragment that resists protease digestion.

Author: Dean, Donald H., Alzate, Oscar, Valaitis, Algimantas P., Audtho, Mongkon
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
United States, Methods, Usage, Proteases, Bacterial toxins, Control, Protein engineering, Mutagenesis, Gypsy moth, Gypsy moths

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Subjects list: Research
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