Abstracts

Biological sciences

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Scarce evidence of yogurt lactic acid bacteria in human feces after daily yogurt consumption by healthy volunteers

Article Abstract:

The double-blind prospective study that includes 114 healthy young volunteers, the presence in human feces of the yogurt organisms Lactobacillus delbrueckii and streptococcus thermophilus after repeated yogurt consumption by culture, specific PCR and DNA hybridation is studied. It was found that the detection of yogurt lactic acid bacteria in total fecal DNA by bacterial culture and PCR assay was consistently negative.

Bacterial genetics

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Lactobacillus rhamnosus strain GG reduces aflatoxin [B.sub.1] transport, metabolism, and toxicity in Caco-2 cells

Article Abstract:

The potential of Lactobacillus rhamnosus GG to reduce aflatoxin [B.sub.1] (AF[B.sub.1]) availability in vitro in Caco-2 cells was investigated. Results suggested that (AF[B.sub.1]) transport can be reduced by GG binding in the Caco-2 model, while these Caco-2 cells can be used to estimate the intestinal metabolism and toxicity of (AF[B.sub.1]) by use of TER measurements.

DNA damage, Aflatoxins

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Acid tolerance of Streptococcus macedonicus as assessed by flow cytometry and single-cell sorting

Article Abstract:

An in situ flow cytometric viability assay, which utilizes carboxyfluorescein diacetate and propidium iodide, is used to identify Streptococcus macedonicus acid tolerance phenotypes. The analysis has shown that the culturability of subpopulations with identical fluorescence characteristics is dependent on the treatments imposed to the cells prior to acid challenge.

Usage, Flow cytometry, Acids, Chemical properties

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