Sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1 and the purification of a biosynthetic intermediate

Article Abstract:

The sequence of pqqC'BA in the genomic region of Methylobacterium extorquens AM1 and the purification of an intermediate of PQQ synthesis were described. Two open reading frames found in the sequenced region of Methylobacterium extorquens were pqqE and pqqC/D. The pqqC mutant produced an intermediate of PQQ biosynthesis which was converted to PQQ using a crude extract of E. coli cells. The intermediate was found in both the culture medium and crude extract.

Analysis, Biosynthesis

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pqqA is not required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1

Article Abstract:

A research was conducted to determine the need for pqqA in the biosynthesis of pyrroloquinoline quinone (PQQ) in Methylobacterium extorquens AM1. pqqA is believed to play a role in the production of PQQ as well as the encoding of PQQ's peptide precusor. Results show that the pqqA can be replaced with a different peptide in pqqA mutants. In addition, the normal methanol induction of PQQ synthesis requires methanol oxidation transcriptional regulator gene.

Genetic aspects

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An RNA polymerase preparation from Methylobacterium extorquens AM1 capable of transcribing from a methylotrophic promoter

Article Abstract:

The purification and characterization of RNA polymerase derived from Methylobacterium extorquens AM1 was described in an experiment. The AM1 cells used for the study were grown either on succinate or on methanol and N-terminal sequence analysis was performed. Test results illustrate the capability of the purified holoenzyme to initiate specifically at a methylotrophic promoter in vitro.

RNA polymerases

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