Soybean chromosome painting: a strategy for somatic cytogenetics

Article Abstract:

Fluorescent in situ hybridization, PCR-primed in situ labeling, and molecular probes have been developed and used to identify and evolve DNA sequences for cytological identification and physical mapping of soybean somatic chromosomes. Use of different cloned examples of each family allows selective labelling of unique subsets of soybean chromosomes.Double hybridization with biotin and digoxigenin determine the overlap in the chromosomes between different probes. It is essential to use different procedures for single-copy and highly repetitive DNA sequences.

Innovations, Identification and classification, Cytogenetics, Chromosomes, Genetic research

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Genetic independence of the nodulation-response gene loci - Rj1, Rj2, and Rj4 - in soybean

Article Abstract:

Hybridization analyses designed to identify linkage or clustering of genes in soybeans were carried out to study the segregation patterns of three genes regulating nodulation in the plant. These are Rj1, Rj2 and Rj4. The dominant and recessive allels of each gene were assayed. The genes were then paired and the chi-square value of linkage for each pair determined. No evidence favoring linkage batween any pair was established.

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Soybean genetic map of RAPD markers assigned to an existing scaffold RFLP map

Article Abstract:

A linkage map of Glycine max was formed by anchoring random amplified polymorphic DNA markers to an existing restriction fragment length polymorphism map.

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