Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

Article Abstract:

A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides and B. thuringiensis was obtained. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species. Findings indicate that the gene for neutral protease has been highly conserved during the evolution of the B. cereus group species.

Proteases, Microbial enzymes, Bacillus cereus

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Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains

Article Abstract:

The utilization of arbitrary primer polymerase chain reaction (AP-PCR) technology for distinguishing commercial products containing Bacillus thuringiensis strains, discriminating known serovars and the differentiation ofthese strains from Bacillus cereus is discussed. Fingerprints of these genomes were provided without any prior sequence formation. The utility of the technique for environmental assessment and quality control was demonstrated.

Methods, Bacillus thuringiensis

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Microarray analysis of Escherichia coli strains from interstitial beach waters of Lake Huron (Canada)

Article Abstract:

DNA microarray analyses of clusters of Escherichia coli strains isolated from interstitial Lake Huron beach water samples are reported. The lake isolates were found to be nonpathogenic and contained tetracycline resistance genes.

Canada, Science & research, Usage, Genetic aspects, Drug resistance in microorganisms, Microbial drug resistance, Escherichia coli, DNA microarrays

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