Strategies for achieving high-level expression of genes in Escherichia coli
Article Abstract:
Efficient gene expression in Escherichia coli needs a strong and precisely regulated promoter at the 5' region of the gene and an efficient transcription terminator positioned at the 3' end. A wide choice of these elements is available to suit the individual need for the expression of different genes. Host strains with low proteolytic activity are also available. Better knowledge of protein secretion by the bacteria can help in secretion of the expressed protein into the medium. Varying the codon usage and use of chaperones can improve gene expression.
Publication Name: Microbiological Reviews
Subject: Biological sciences
ISSN: 0146-0749
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Central roles of small GTPases in the development of cell polarity in yeast and beyond
Article Abstract:
The molecular mechanisms underlying the development of cell polarity in budding yeast is reviewed with particular focus on the roles of small GTPases. The key signaling pathways that regulate cell polarization during the mitotic cell cycle and during mating is discussed.
Publication Name: Microbiology and Molecular Biology Reviews
Subject: Biological sciences
ISSN: 1092-2172
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
Allosteric regulation of catalytic activity: Escherichia coli aspartate transcarbamoylase versus yeast chorismate mutase
Article Abstract:
This review focuses on the major investigations into the baker's yeast made during the last decade. The comparison of the established aspartate transcarbamoylase model system with the catalytic and regulatory features of E. coli has been performed and analyzed.
Publication Name: Microbiology and Molecular Biology Reviews
Subject: Biological sciences
ISSN: 1092-2172
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli. Impact of rpoS deletion on Escherichia coli biofilms
- Abstracts: Comparative survival of free Shiga toxin 2-encoding phages and Escherichia coli strains outside the gut
- Abstracts: Statistical method for estimating the effective population size in Pacific salmon. Salmon: A new autosomal mutation demostrating incomplete dominance in the Boine snake Boa Constrictor
- Abstracts: Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils
- Abstracts: Scanning electron and light microscopic study of microbial succession on Bethlehem St. Nectaire cheese. Microarray analysis of microbial virulence factors