Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity
Article Abstract:
The structure and activity of the Pyrococcus furiosus's flap endonuclease 1 (FEN-1) were studied to determine its role in DNA repair and replication. The crystal structure of FEN-1 exhibited interactions for the single- and double-stranded portions of the flap DNA substrate. The binding of the flap DNA substrates' stranded regions were influenced by two structural motifs. It was revealed that the conserved C terminus of FEN-1 influenced its involvement in the DNA replication fork apparatus through the interaction with the C-terminal face of the proliferating cell nuclear antigen.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
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Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis
Article Abstract:
A study was conducted to determine the atomic structure of Escherichia coli endonuclease IV both alone and in complex with duplex DNA containing a synthetic abasic site. Results provide information on the structures of the Endo IV apurinic/apyrimidinic enduclease family and the DNA-bound structures for a DNA repair enzyme. These structures reveal a new use of alpha8beta8 TIM barrel fold for DNA binding and identify key structural features responsible for DNA damage recognition and catalysis.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair
Article Abstract:
Flap EndoNuclease-1 (FEN-1) is a structure-specific nuclease that is central to both DNA replication and repair processes. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA (proliferating cell nuclear anti-clamp) alignments are discussed.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2004
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